Publications
Structure-based discovery of conformationally selective inhibitors of the serotonin transporter
Isha Singh, Anubha Seth, Christian B. Billesbølle, Joao Braz, Ramona M. Rodriguiz, Kasturi Roy, Bethlehem Bekele, Veronica Craik, Xi-Ping Huang, Danila Boytsov, Vladimir M. Pogorelov, Parnian Lak, Henry O’Donnell, Walter Sandtner, John J. Irwin, Bryan L. Roth, Allan I. Basbaum, William C. Wetsel, Aashish Manglik, Brian K. Shoichet, and Gary Rudnick
Published in Cell.
Structural basis of odorant recognition by a human odorant receptor
Christian B. Billesbølle, Claire A. de March, Wijnand J. C. van der Velden, Ning Ma, Jeevan Tewari, Claudia Llinas del Torrent, Linus Li, Bryan Faust, Nagarajan Vaidehi, Hiroaki Matsunami, and Aashish Manglik
Published in Nature.
Discrete GPCR-triggered endocytic modes enable β-arrestins to flexibly regulate cell signaling
Benjamin Barsi-Rhyne, Aashish Manglik, and Mark von Zastrow
Published in eLife.
Autoantibody mimicry of hormone action at the thyrotropin receptor
Bryan Faust, Christian B. Billesbølle, Carl-Mikael Suomivuori, Isha Singh, Kaihua Zhang, Nicholas Hoppe, Antonio F. M. Pinto, Jolene K. Diedrich, Yagmur Muftuoglu, Mariusz W. Szkudlinski, Alan Saghatelian, Ron O. Dror, Yifan Cheng, and Aashish Manglik
Published in Nature.
Selective G protein signaling driven by substance P–neurokinin receptor dynamics
Julian A. Harris, Bryan Faust, Arisbel B. Gondin, Marc André Dämgen, Carl-Mikael Suomivuori, Nicholas A. Veldhuis, Yifan Cheng, Ron O. Dror, David M. Thal, and Aashish Manglik
Published in Nature Chemical Biology.
Biochemical Assays to Directly Assess Smoothened Activation by a Conformationally Sensitive Nanobody
Ishan Deshpande and Aashish Manglik
Published in Hedgehog Signaling.
Structural insight into SARS-CoV-2 neutralizing antibodies and modulation of syncytia
Daniel Asarnow, Bei Wang, Wen-Hsin Lee, Yuanyu Hu, Ching-Wen Huang, Bryan Faust, Patricia Miang Lon Ng, Eve Zi Xian Ngoh, Markus Bohn, David Bulkley, Andrés Pizzorno, Beatrice Ary, Hwee Ching Tan, Chia Yin Lee, Rabiatul Adawiyah Minhat, Olivier Terrier, Mun Kuen Soh, Frannie Jiuyi Teo, Yvonne Yee Chin Yeap, Shirley Gek Kheng Seah, Conrad En Zuo Chan, Emily Connelly, Nicholas J. Young, Sebastian Maurer-Stroh, Laurent Renia, Brendon John Hanson, Manuel Rosa-Calatrava, Aashish Manglik, Yifan Cheng, Charles S. Craik, and Cheng-I Wang
Published in Cell.
Surface Proteomics Reveals CD72 as a Target for In Vitro–Evolved Nanobody-Based CAR-T Cells in KMT2A/MLL1-Rearranged B-ALL
Matthew A. Nix, Kamal Mandal, Huimin Geng, Neha Paranjape, Yu-Hsiu T. Lin, Jose M. Rivera, Makeba Marcoulis, Kristie L. White, Jeffrey D. Whitman, Sagar P. Bapat, Kevin R. Parker, Jonathan Ramirez, Anne Deucher, Paul Phojanokong, Veronica Steri, Faranak Fattahi, Byron C. Hann, Ansuman T. Satpathy, Aashish Manglik, Elliot Stieglitz, and Arun P. Wiita
Published in Cancer Discovery.
CryoEM and AI reveal a structure of SARS-CoV-2 Nsp2, a multifunctional protein involved in key host processes
Meghna Gupta, Caleigh M. Azumaya, Michelle Moritz, Sergei Pourmal, Amy Diallo, Gregory E. Merz, Gwendolyn Jang, Mehdi Bouhaddou, Andrea Fossati, Axel F. Brilot, Devan Diwanji, Evelyn Hernandez, Nadia Herrera, Huong T. Kratochvil, Victor L. Lam, Fei Li, Yang Li, Henry C. Nguyen, Carlos Nowotny, Tristan W. Owens, Jessica K. Peters, Alexandrea N. Rizo, Ursula Schulze-Gahmen, Amber M. Smith, Iris D. Young, Zanlin Yu, Daniel Asarnow, Christian Billesbølle, Melody G. Campbell, Jen Chen, Kuei-Ho Chen, Un Seng Chio, Miles Sasha Dickinson, Loan Doan, Mingliang Jin, Kate Kim, Junrui Li, Yen-Li Li, Edmond Linossi, Yanxin Liu, Megan Lo, Jocelyne Lopez, Kyle E. Lopez, Adamo Mancino, Frank R. Moss III, Michael D. Paul, Komal Ishwar Pawar, Adrian Pelin, Thomas H. Pospiech, Jr., Cristina Puchades, Soumya Govinda Remesh, Maliheh Safari, Kaitlin Schaefer, Ming Sun, Mariano C Tabios, Aye C. Thwin, Erron W. Titus, Raphael Trenker, Eric Tse, Tsz Kin Martin Tsui, Feng Wang, Kaihua Zhang, Yang Zhang, Jianhua Zhao, Fengbo Zhou, Yuan Zhou, Lorena Zuliani-Alvarez, QCRG Structural Biology Consortium, David A Agard, Yifan Cheng, James S Fraser, Natalia Jura, Tanja Kortemme, Aashish Manglik, Daniel R. Southworth, Robert M Stroud, Danielle L Swaney, Nevan J Krogan, Adam Frost, Oren S Rosenberg, and Kliment A Verba
Published in bioRxiv.
Protein design and variant prediction using autoregressive generative models
Jung-Eun Shin, Adam J. Riesselman, Aaron W. Kollasch, Conor McMahon, Elana Simon, Chris Sander, Aashish Manglik, Andrew C. Kruse, and Debora S. Marks
Published in Nature Communications.
Smoothened transduces Hedgehog signals via activity-dependent sequestration of PKA catalytic subunits
Corvin D. Arveseth, John T. Happ, Danielle S. Hedeen, Ju-Fen Zhu, Jacob L. Capener, Dana Klatt Shaw, Ishan Deshpande, Jiahao Liang, Jiewei Xu, Sara L. Stubben, Isaac B. Nelson, Madison F. Walker, Kouki Kawakami, Asuka Inoue, Nevan J. Krogan, David J. Grunwald, Ruth Hüttenhain, Aashish Manglik, and Benjamin R. Myers
Published in PLOS Biology.
An ultrapotent synthetic nanobody neutralizes SARS-CoV-2 by stabilizing inactive Spike
Michael Schoof, Bryan Faust, Reuben A. Saunders, Smriti Sangwan, Veronica Rezelj, Nick Hoppe, Morgane Boone, Christian B. Billesbølle, Cristina Puchades, Caleigh M. Azumaya, Huong T. Kratochvil, Marcell Zimanyi, Ishan Deshpande, Jiahao Liang, Sasha Dickinson, Henry C. Nguyen, Cynthia M. Chio, Gregory E. Merz, Michael C. Thompson, Devan Diwanji, Kaitlin Schaefer, Aditya A. Anand, Niv Dobzinski, Beth Shoshana Zha, Camille R. Simoneau, Kristoffer Leon, Kris M. White, Un Seng Chio, Meghna Gupta, Mingliang Jin, Fei Li, Yanxin Liu, Kaihua Zhang, David Bulkley, Ming Sun, Amber M. Smith, Alexandrea N. Rizo, Frank Moss, Axel F. Brilot, Sergei Pourmal, Raphael Trenker, Thomas Pospiech, Sayan Gupta, Benjamin Barsi-Rhyne, Vladislav Belyy, Andrew W. Barile-Hill, Silke Nock, Yuwei Liu, Nevan J. Krogan, Corie Y. Ralston, Danielle L. Swaney, Adolfo García-Sastre, Melanie Ott, Marco Vignuzzi, QCRG Structural Biology Consortium, Peter Walter, Aashish Manglik, Caleigh M. Azumaya, Cristina Puchades, Ming Sun, Julian R. Braxton, Axel F. Brilot, Meghna Gupta, Fei Li, Kyle E. Lopez, Arthur Melo, Gregory E. Merz, Frank Moss, Joana Paulino, Thomas H. Pospiech, Sergei Pourmal, Alexandrea N. Rizo, Amber M. Smith, Paul V. Thomas, Feng Wang, Zanlin Yu, Miles Sasha Dickinson, Henry C. Nguyen, Daniel Asarnow, Melody G. Campbell, Cynthia M. Chio, Un Seng Chio, Devan Diwanji, Bryan Faust, Meghna Gupta, Nick Hoppe, Mingliang Jin, Junrui Li, Yanxin Liu, Gregory E. Merz, Smriti Sangwan, Tsz Kin Martin Tsui, Raphael Trenker, Donovan Trinidad, Eric Tse, Kaihua Zhang, Fengbo Zhou, Nadia Herrera, Huong T. Kratochvil, Ursula Schulze-Gahmen, Michael C. Thompson, Iris D. Young, Justin Biel, Ishan Deshpande, Xi Liu, Christian Bache Billesbølle, Carlos Nowotny, Amber M. Smith, Jianhua Zhao, Alisa Bowen, Nick Hoppe, Yen-Li Li, Phuong Nguyen, Mali Safari, Kaitlin Schaefer, Natalie Whitis, Michelle Moritz, Tristan W. Owens, Amy Diallo, Kate Kim, Jessica K. Peters, Erron W. Titus, Jenny Chen, Loan Doan, Sebastian Flores, Victor L. Lam, Yang Li, Megan Lo, Aye C. Thwin, Stephanie Wankowicz, Yang Zhang, David Bulkley, Arceli Joves, Almarie Joves, Liam McKay, Mariano Tabios, Oren S. Rosenberg, Kliment A. Verba, David A. Agard, Yifan Cheng, James S. Fraser, Adam Frost, Natalia Jura, Tanja Kortemme, Nevan J. Krogan, Aashish Manglik, Daniel R. Southworth, and Robert M. Stroud
Published in Science.
Hedgehog pathway activation through nanobody-mediated conformational blockade of the Patched sterol conduit
Yunxiao Zhang, Wan-Jin Lu, David P. Bulkley, Jiahao Liang, Arthur Ralko, Shuo Han, Kelsey J. Roberts, Anping Li, Wonhwa Cho, Yifan Cheng, Aashish Manglik, and Philip A. Beachy
Published in Proceedings of the National Academy of Sciences.
Universal Polymerase Chain Reaction and Antibody Testing Demonstrate Little to No Transmission of Severe Acute Respiratory Syndrome Coronavirus 2 in a Rural Community
Ayesha Appa, Saki Takahashi, Isabel Rodriguez-Barraquer, Gabriel Chamie, Aenor Sawyer, Elias Duarte, Jill Hakim, Keirstinne Turcios, Joanna Vinden, Owen Janson, Aashish Manglik, Michael J Peluso, Steven G Deeks, Timothy J Henrich, Leonel Torres, Mary Rodgers, John Hackett, Charles Chiu, Diane Havlir, and Bryan Greenhouse
Published in Open Forum Infectious Diseases.
Bi-paratopic and multivalent VH domains block ACE2 binding and neutralize SARS-CoV-2
Colton J. Bracken, Shion A. Lim, Paige Solomon, Nicholas J. Rettko, Duy P. Nguyen, Beth Shoshana Zha, Kaitlin Schaefer, James R. Byrnes, Jie Zhou, Irene Lui, Jia Liu, Katarina Pance, QCRG Structural Biology Consortium, Xin X. Zhou, Kevin K. Leung, and James A. Wells
Published in Nature Chemical Biology.
Comparative host-coronavirus protein interaction networks reveal pan-viral disease mechanisms
David E. Gordon, Joseph Hiatt, Mehdi Bouhaddou, Veronica V. Rezelj, Svenja Ulferts, Hannes Braberg, Alexander S. Jureka, Kirsten Obernier, Jeffrey Z. Guo, Jyoti Batra, Robyn M. Kaake, Andrew R. Weckstein, Tristan W. Owens, Meghna Gupta, Sergei Pourmal, Erron W. Titus, Merve Cakir, Margaret Soucheray, Michael McGregor, Zeynep Cakir, Gwendolyn Jang, Matthew J. O’Meara, Tia A. Tummino, Ziyang Zhang, Helene Foussard, Ajda Rojc, Yuan Zhou, Dmitry Kuchenov, Ruth Hüttenhain, Jiewei Xu, Manon Eckhardt, Danielle L. Swaney, Jacqueline M. Fabius, Manisha Ummadi, Beril Tutuncuoglu, Ujjwal Rathore, Maya Modak, Paige Haas, Kelsey M. Haas, Zun Zar Chi Naing, Ernst H. Pulido, Ying Shi, Inigo Barrio-Hernandez, Danish Memon, Eirini Petsalaki, Alistair Dunham, Miguel Correa Marrero, David Burke, Cassandra Koh, Thomas Vallet, Jesus A. Silvas, Caleigh M. Azumaya, Christian Billesbølle, Axel F. Brilot, Melody G. Campbell, Amy Diallo, Miles Sasha Dickinson, Devan Diwanji, Nadia Herrera, Nick Hoppe, Huong T. Kratochvil, Yanxin Liu, Gregory E. Merz, Michelle Moritz, Henry C. Nguyen, Carlos Nowotny, Cristina Puchades, Alexandrea N. Rizo, Ursula Schulze-Gahmen, Amber M. Smith, Ming Sun, Iris D. Young, Jianhua Zhao, Daniel Asarnow, Justin Biel, Alisa Bowen, Julian R. Braxton, Jen Chen, Cynthia M. Chio, Un Seng Chio, Ishan Deshpande, Loan Doan, Bryan Faust, Sebastian Flores, Mingliang Jin, Kate Kim, Victor L. Lam, Fei Li, Junrui Li, Yen-Li Li, Yang Li, Xi Liu, Megan Lo, Kyle E. Lopez, Arthur A. Melo, Frank R. Moss, Phuong Nguyen, Joana Paulino, Komal Ishwar Pawar, Jessica K. Peters, Thomas H. Pospiech, Maliheh Safari, Smriti Sangwan, Kaitlin Schaefer, Paul V. Thomas, Aye C. Thwin, Raphael Trenker, Eric Tse, Tsz Kin Martin Tsui, Feng Wang, Natalie Whitis, Zanlin Yu, Kaihua Zhang, Yang Zhang, Fengbo Zhou, Daniel Saltzberg, QCRG Structural Biology Consortium, Anthony J. Hodder, Amber S. Shun-Shion, Daniel M. Williams, Kris M. White, Romel Rosales, Thomas Kehrer, Lisa Miorin, Elena Moreno, Arvind H. Patel, Suzannah Rihn, Mir M. Khalid, Albert Vallejo-Gracia, Parinaz Fozouni, Camille R. Simoneau, Theodore L. Roth, David Wu, Mohd Anisul Karim, Maya Ghoussaini, Ian Dunham, Francesco Berardi, Sebastian Weigang, Maxime Chazal, Jisoo Park, James Logue, Marisa McGrath, Stuart Weston, Robert Haupt, C. James Hastie, Matthew Elliott, Fiona Brown, Kerry A. Burness, Elaine Reid, Mark Dorward, Clare Johnson, Stuart G. Wilkinson, Anna Geyer, Daniel M. Giesel, Carla Baillie, Samantha Raggett, Hannah Leech, Rachel Toth, Nicola Goodman, Kathleen C. Keough, Abigail L. Lind, Zoonomia Consortium, Reyna J. Klesh, Kafi R. Hemphill, Jared Carlson-Stevermer, Jennifer Oki, Kevin Holden, Travis Maures, Katherine S. Pollard, Andrej Sali, David A. Agard, Yifan Cheng, James S. Fraser, Adam Frost, Natalia Jura, Tanja Kortemme, Aashish Manglik, Daniel R. Southworth, Robert M. Stroud, Dario R. Alessi, Paul Davies, Matthew B. Frieman, Trey Ideker, Carmen Abate, Nolwenn Jouvenet, Georg Kochs, Brian Shoichet, Melanie Ott, Massimo Palmarini, Kevan M. Shokat, Adolfo García-Sastre, Jeremy A. Rassen, Robert Grosse, Oren S. Rosenberg, Kliment A. Verba, Christopher F. Basler, Marco Vignuzzi, Andrew A. Peden, Pedro Beltrao, Nevan J. Krogan, Tristan W. Owens, Meghna Gupta, Sergei Pourmal, Erron W. Titus, Caleigh M. Azumaya, Christian Billesbølle, Axel F. Brilot, Melody G. Campbell, Amy Diallo, Miles Sasha Dickinson, Devan Diwanji, Nadia Herrera, Nick Hoppe, Huong T. Kratochvil, Yanxin Liu, Gregory E. Merz, Michelle Moritz, Henry C. Nguyen, Carlos Nowotny, Cristina Puchades, Alexandrea N. Rizo, Ursula Schulze-Gahmen, Amber M. Smith, Ming Sun, Iris D. Young, Jianhua Zhao, Daniel Asarnow, Justin Biel, Alisa Bowen, Julian R. Braxton, Jen Chen, Cynthia M. Chio, Un Seng Chio, Ishan Deshpande, Loan Doan, Bryan Faust, Sebastian Flores, Mingliang Jin, Kate Kim, Victor L. Lam, Fei Li, Junrui Li, Yen-Li Li, Yang Li, Xi Liu, Megan Lo, Kyle E. Lopez, Arthur A. Melo, Frank R. Moss, Phuong Nguyen, Joana Paulino, Komal Ishwar Pawar, Jessica K. Peters, Thomas H. Pospiech, Maliheh Safari, Smriti Sangwan, Kaitlin Schaefer, Paul V. Thomas, Aye C. Thwin, Raphael Trenker, Eric Tse, Tsz Kin Martin Tsui, Feng Wang, Natalie Whitis, Zanlin Yu, Kaihua Zhang, Yang Zhang, Fengbo Zhou, Donovan Trinidad, David A. Agard, Yifan Cheng, James S. Fraser, Adam Frost, Natalia Jura, Tanja Kortemme, Aashish Manglik, Daniel R. Southworth, Robert M. Stroud, Oren S. Rosenberg, Kliment A. Verba, Joana Damas, Graham M. Hughes, Kathleen C. Keough, Corrie A. Painter, Nicole S. Persky, Marco Corbo, Bodgan Kirilenko, Michael Hiller, Klaus-Peter Koepfli, Irene Kaplow, Morgan Wirthlin, Andreas R. Pfenning, Huabin Zhao, Diane P. Genereux, Ross Swofford, Abigail Lind, Katherine S. Pollard, Oliver A. Ryderq, Martin T. Nweeia, Jennifer Meadows, Michael Dong, Ola Wallerman, Vikki Marinescu, Kerstin Lindblad-Toh, David A. Ray, Sarahjane Power, Emma C. Teeling, Gaurav Chauhan, Shirley Xue Li, Elinor K. Karlsson, and Harris A. Lewin
Published in Science.
Structure of hepcidin-bound ferroportin reveals iron homeostatic mechanisms
Christian B. Billesbølle, Caleigh M. Azumaya, Rachael C. Kretsch, Alexander S. Powers, Shane Gonen, Simon Schneider, Tara Arvedson, Ron O. Dror, Yifan Cheng, and Aashish Manglik
Published in Nature.
β-Arrestin–Biased Angiotensin II Receptor Agonists for COVID-19
Aashish Manglik, Laura M. Wingler, Howard A. Rockman, and Robert J. Lefkowitz
Published in Circulation.
Test performance evaluation of SARS-CoV-2 serological assays
Jeffrey D. Whitman, Joseph Hiatt, Cody T. Mowery, Brian R. Shy, Ruby Yu, Tori N. Yamamoto, Ujjwal Rathore, Gregory M. Goldgof, Caroline Whitty, Jonathan M. Woo, Antonia E. Gallman, Tyler E. Miller, Andrew G. Levine, David N. Nguyen, Sagar P. Bapat, Joanna Balcerek, Sophia A. Bylsma, Ana M. Lyons, Stacy Li, Allison Wai-yi Wong, Eva Mae Gillis-Buck, Zachary B. Steinhart, Youjin Lee, Ryan Apathy, Mitchell J. Lipke, Jennifer Anne Smith, Tina Zheng, Ian C. Boothby, Erin Isaza, Jackie Chan, Dante D. Acenas, Jinwoo Lee, Trisha A. Macrae, Than S. Kyaw, David Wu, Dianna L. Ng, Wei Gu, Vanessa A. York, Haig Alexander Eskandarian, Perri C. Callaway, Lakshmi Warrier, Mary E. Moreno, Justine Levan, Leonel Torres, Lila A. Farrington, Rita Loudermilk, Kanishka Koshal, Kelsey C. Zorn, Wilfredo F. Garcia-Beltran, Diane Yang, Michael G. Astudillo, Bradley E. Bernstein, Jeffrey A. Gelfand, Edward T. Ryan, Richelle C. Charles, A. John Iafrate, Jochen K. Lennerz, Steve Miller, Charles Y. Chiu, Susan L. Stramer, Michael R. Wilson, Aashish Manglik, Chun Jimmie Ye, Nevan J. Krogan, Mark S. Anderson, Jason G. Cyster, Joel D. Ernst, Alan H. B. Wu, Kara L. Lynch, Caryn Bern, Patrick D. Hsu, and Alexander Marson
Published in medRxiv.
Agonist-selective recruitment of engineered protein probes and of GRK2 by opioid receptors in living cells
Miriam Stoeber, Damien Jullié, Joy Li, Soumen Chakraborty, Susruta Majumdar, Nevin A Lambert, Aashish Manglik, and Mark von Zastrow
Published in eLife.
Molecular Basis of Opioid Action: From Structures to New Leads
Aashish Manglik
Published in Biological Psychiatry.
Smoothened stimulation by membrane sterols drives Hedgehog pathway activity
Ishan Deshpande, Jiahao Liang, Danielle Hedeen, Kelsey J. Roberts, Yunxiao Zhang, Betty Ha, Naomi R. Latorraca, Bryan Faust, Ron O. Dror, Philip A. Beachy, Benjamin R. Myers, and Aashish Manglik
Published in Nature.
Development of an antibody fragment that stabilizes GPCR/G-protein complexes
Shoji Maeda, Antoine Koehl, Hugues Matile, Hongli Hu, Daniel Hilger, Gebhard F. X. Schertler, Aashish Manglik, Georgios Skiniotis, Roger J. P. Dawson, and Brian K. Kobilka
Published in Nature Communications.
Measuring ligand efficacy at the mu-opioid receptor using a conformational biosensor
Kathryn E Livingston, Jacob P Mahoney, Aashish Manglik, Roger K Sunahara, and John R Traynor
Published in eLife.
Structure of the µ-opioid receptor–Gi protein complex
Antoine Koehl, Hongli Hu, Shoji Maeda, Yan Zhang, Qianhui Qu, Joseph M. Paggi, Naomi R. Latorraca, Daniel Hilger, Roger Dawson, Hugues Matile, Gebhard F. X. Schertler, Sebastien Granier, William I. Weis, Ron O. Dror, Aashish Manglik, Georgios Skiniotis, and Brian K. Kobilka
Published in Nature.
A Genetically Encoded Biosensor Reveals Location Bias of Opioid Drug Action
Miriam Stoeber, Damien Jullié, Braden T. Lobingier, Toon Laeremans, Jan Steyaert, Peter W. Schiller, Aashish Manglik, and Mark von Zastrow
Published in Neuron.
Sortase ligation enables homogeneous GPCR phosphorylation to reveal diversity in β-arrestin coupling
Dean P. Staus, Laura M. Wingler, Minjung Choi, Biswaranjan Pani, Aashish Manglik, Andrew C. Kruse, and Robert J. Lefkowitz
Published in Proceedings of the National Academy of Sciences.
Yeast surface display platform for rapid discovery of conformationally selective nanobodies
Conor McMahon, Alexander S. Baier, Roberta Pascolutti, Marcin Wegrecki, Sanduo Zheng, Janice X. Ong, Sarah C. Erlandson, Daniel Hilger, Søren G. F. Rasmussen, Aaron M. Ring, Aashish Manglik, and Andrew C. Kruse
Published in Nature Structural & Molecular Biology.
Structural Basis for G Protein-Coupled Receptor Activation
Aashish Manglik and Andrew C. Kruse
Published in Biochemistry.
Nanobodies to Study G Protein–Coupled Receptor Structure and Function
Aashish Manglik, Brian K. Kobilka, and Jan Steyaert
Published in Annual Review of Pharmacology and Toxicology.
Structure-based discovery of opioid analgesics with reduced side effects
Aashish Manglik, Henry Lin, Dipendra K. Aryal, John D. McCorvy, Daniela Dengler, Gregory Corder, Anat Levit, Ralf C. Kling, Viachasl Bernat, Harald Hübner, Xi-Ping Huang, Maria F. Sassano, Patrick M. Giguère, Stefan Löber, Da Duan, Grégory Scherrer, Brian K. Kobilka, Peter Gmeiner, Bryan L. Roth, and Brian K. Shoichet
Published in Nature.
Allosteric nanobodies reveal the dynamic range and diverse mechanisms of G-protein-coupled receptor activation
Dean P. Staus, Ryan T. Strachan, Aashish Manglik, Biswaranjan Pani, Alem W. Kahsai, Tae Hun Kim, Laura M. Wingler, Seungkirl Ahn, Arnab Chatterjee, Ali Masoudi, Andrew C. Kruse, Els Pardon, Jan Steyaert, William I. Weis, R. Scott Prosser, Brian K. Kobilka, Tommaso Costa, and Robert J. Lefkowitz
Published in Nature.
Allosteric coupling from G protein to the agonist-binding pocket in GPCRs
Brian T. DeVree, Jacob P. Mahoney, Gisselle A. Vélez-Ruiz, Soren G. F. Rasmussen, Adam J. Kuszak, Elin Edwald, Juan-Jose Fung, Aashish Manglik, Matthieu Masureel, Yang Du, Rachel A. Matt, Els Pardon, Jan Steyaert, Brian K. Kobilka, and Roger K. Sunahara
Published in Nature.
Crystal structure of the human σ1 receptor
Hayden R. Schmidt, Sanduo Zheng, Esin Gurpinar, Antoine Koehl, Aashish Manglik, and Andrew C. Kruse
Published in Nature.
High-density grids for efficient data collection from multiple crystals
Elizabeth L. Baxter, Laura Aguila, Roberto Alonso-Mori, Christopher O. Barnes, Christopher A. Bonagura, Winnie Brehmer, Axel T. Brunger, Guillermo Calero, Tom T. Caradoc-Davies, Ruchira Chatterjee, William F. Degrado, James S. Fraser, Mohamed Ibrahim, Jan Kern, Brian K. Kobilka, Andrew C. Kruse, Karl M. Larsson, Heinrik T. Lemke, Artem Y. Lyubimov, Aashish Manglik, Scott E. McPhillips, Erik Norgren, Siew S. Pang, S. M. Soltis, Jinhu Song, Jessica Thomaston, Yingssu Tsai, William I. Weis, Rahel A. Woldeyes, Vittal Yachandra, Junko Yano, Athina Zouni, and Aina E. Cohen
Published in Acta Crystallographica Section D Structural Biology.
Engineering high-affinity PD-1 variants for optimized immunotherapy and immuno-PET imaging
Roy L. Maute, Sydney R. Gordon, Aaron T. Mayer, Melissa N. McCracken, Arutselvan Natarajan, Nan Guo Ring, Richard Kimura, Jonathan M. Tsai, Aashish Manglik, Andrew C. Kruse, Sanjiv S. Gambhir, Irving L. Weissman, and Aaron M. Ring
Published in Proceedings of the National Academy of Sciences.
Structural insights into µ-opioid receptor activation
Weijiao Huang, Aashish Manglik, A. J. Venkatakrishnan, Toon Laeremans, Evan N. Feinberg, Adrian L. Sanborn, Hideaki E. Kato, Kathryn E. Livingston, Thor S. Thorsen, Ralf C. Kling, Sébastien Granier, Peter Gmeiner, Stephen M. Husbands, John R. Traynor, William I. Weis, Jan Steyaert, Ron O. Dror, and Brian K. Kobilka
Published in Nature.
Propagation of conformational changes during μ-opioid receptor activation
Rémy Sounier, Camille Mas, Jan Steyaert, Toon Laeremans, Aashish Manglik, Weijiao Huang, Brian K. Kobilka, Héléne Déméné, and Sébastien Granier
Published in Nature.
Structural basis for nucleotide exchange in heterotrimeric G proteins
Ron O. Dror, Thomas J. Mildorf, Daniel Hilger, Aashish Manglik, David W. Borhani, Daniel H. Arlow, Ansgar Philippsen, Nicolas Villanueva, Zhongyu Yang, Michael T. Lerch, Wayne L. Hubbell, Brian K. Kobilka, Roger K. Sunahara, and David E. Shaw
Published in Science.
Structural Insights into the Dynamic Process of β2-Adrenergic Receptor Signaling
Aashish Manglik, Tae Hun Kim, Matthieu Masureel, Christian Altenbach, Zhongyu Yang, Daniel Hilger, Michael T. Lerch, Tong Sun Kobilka, Foon Sun Thian, Wayne L. Hubbell, R. Scott Prosser, and Brian K. Kobilka
Published in Cell.
G Protein-coupled Receptor Kinases of the GRK4 Protein Subfamily Phosphorylate Inactive G Protein-coupled Receptors (GPCRs)
Lingyong Li, Kristoff T. Homan, Sergey A. Vishnivetskiy, Aashish Manglik, John J.G. Tesmer, Vsevolod V. Gurevich, and Eugenia V. Gurevich
Published in Journal of Biological Chemistry.
A comparison of chemical shift sensitivity of trifluoromethyl tags: optimizing resolution in 19F NMR studies of proteins
Libin Ye, Sacha Thierry Larda, Yi Feng Frank Li, Aashish Manglik, and R. Scott Prosser
Published in Journal of Biomolecular NMR.
Goniometer-based femtosecond crystallography with X-ray free electron lasers
Aina E. Cohen, S. Michael Soltis, Ana González, Laura Aguila, Roberto Alonso-Mori, Christopher O. Barnes, Elizabeth L. Baxter, Winnie Brehmer, Aaron S. Brewster, Axel T. Brunger, Guillermo Calero, Joseph F. Chang, Matthieu Chollet, Paul Ehrensberger, Thomas L. Eriksson, Yiping Feng, Johan Hattne, Britt Hedman, Michael Hollenbeck, James M. Holton, Stephen Keable, Brian K. Kobilka, Elena G. Kovaleva, Andrew C. Kruse, Henrik T. Lemke, Guowu Lin, Artem Y. Lyubimov, Aashish Manglik, Irimpan I. Mathews, Scott E. McPhillips, Silke Nelson, John W. Peters, Nicholas K. Sauter, Clyde A. Smith, Jinhu Song, Hilary P. Stevenson, Yingssu Tsai, Monarin Uervirojnangkoorn, Vladimir Vinetsky, Soichi Wakatsuki, William I. Weis, Oleg A. Zadvornyy, Oliver B. Zeldin, Diling Zhu, and Keith O. Hodgson
Published in Proceedings of the National Academy of Sciences.
Covalent agonists for studying G protein-coupled receptor activation
Dietmar Weichert, Andrew C. Kruse, Aashish Manglik, Christine Hiller, Cheng Zhang, Harald Hübner, Brian K. Kobilka, and Peter Gmeiner
Published in Proceedings of the National Academy of Sciences.
The role of protein dynamics in GPCR function: insights from the β2AR and rhodopsin
Aashish Manglik and Brian Kobilka
Published in Current Opinion in Cell Biology.
Activation and allosteric modulation of a muscarinic acetylcholine receptor
Andrew C. Kruse, Aaron M. Ring, Aashish Manglik, Jianxin Hu, Kelly Hu, Katrin Eitel, Harald Hübner, Els Pardon, Celine Valant, Patrick M. Sexton, Arthur Christopoulos, Christian C. Felder, Peter Gmeiner, Jan Steyaert, William I. Weis, K. Christopher Garcia, Jürgen Wess, and Brian K. Kobilka
Published in Nature.
Applications of molecular replacement to G protein-coupled receptors
Andrew C. Kruse, Aashish Manglik, Brian K. Kobilka, and William I. Weis
Published in Acta Crystallographica Section D Biological Crystallography.
Adrenaline-activated structure of β2-adrenoceptor stabilized by an engineered nanobody
Aaron M. Ring, Aashish Manglik, Andrew C. Kruse, Michael D. Enos, William I. Weis, K. Christopher Garcia, and Brian K. Kobilka
Published in Nature.
The Role of Ligands on the Equilibria Between Functional States of a G Protein-Coupled Receptor
Tae Hun Kim, Ka Young Chung, Aashish Manglik, Alexandar L. Hansen, Ron O. Dror, Thomas J. Mildorf, David E. Shaw, Brian K. Kobilka, and R. Scott Prosser
Published in Journal of the American Chemical Society.
Structure of active β-arrestin-1 bound to a G-protein-coupled receptor phosphopeptide
Arun K. Shukla, Aashish Manglik, Andrew C. Kruse, Kunhong Xiao, Rosana I. Reis, Wei-Chou Tseng, Dean P. Staus, Daniel Hilger, Serdar Uysal, Li-Yin Huang, Marcin Paduch, Prachi Tripathi-Shukla, Akiko Koide, Shohei Koide, William I. Weis, Anthony A. Kossiakoff, Brian K. Kobilka, and Robert J. Lefkowitz
Published in Nature.
The Dynamic Process of β2-Adrenergic Receptor Activation
Rie Nygaard, Yaozhong Zou, Ron O. Dror, Thomas J. Mildorf, Daniel H. Arlow, Aashish Manglik, Albert C. Pan, Corey W. Liu, Juan José Fung, Michael P. Bokoch, Foon Sun Thian, Tong Sun Kobilka, David E. Shaw, Luciano Mueller, R. Scott Prosser, and Brian K. Kobilka
Published in Cell.
Role of Detergents in Conformational Exchange of a G Protein-coupled Receptor
Ka Young Chung, Tae Hun Kim, Aashish Manglik, Rohan Alvares, Brian K. Kobilka, and R.Scott Prosser
Published in Journal of Biological Chemistry.
A New Class of Amphiphiles Bearing Rigid Hydrophobic Groups for Solubilization and Stabilization of Membrane Proteins
Pil Seok Chae, Søren G. F. Rasmussen, Rohini R. Rana, Kamil Gotfryd, Andrew C. Kruse, Aashish Manglik, Kyung Ho Cho, Shailika Nurva, Ulrik Gether, Lan Guan, Claus J. Loland, Bernadette Byrne, Brian K. Kobilka, and Samuel H. Gellman
Published in Chemistry – A European Journal.
Structure of the δ-opioid receptor bound to naltrindole
Sébastien Granier, Aashish Manglik, Andrew C. Kruse, Tong Sun Kobilka, Foon Sun Thian, William I. Weis, and Brian K. Kobilka
Published in Nature.
Crystal structure of the µ-opioid receptor bound to a morphinan antagonist
Aashish Manglik, Andrew C. Kruse, Tong Sun Kobilka, Foon Sun Thian, Jesper M. Mathiesen, Roger K. Sunahara, Leonardo Pardo, William I. Weis, Brian K. Kobilka, and Sébastien Granier
Published in Nature.
Stably Integrated luxCDABE for Assessment of Salmonella Invasion Kinetics
Kelly N. Flentie, Min Qi, Seth T. Gammon, Yasmin Razia, Felix Lui, Luciano Marpegan, Aashish Manglik, David Piwnica-Worms, and Jeffrey S. McKinney
Published in Molecular Imaging.