Posts by Year

2023

Structure-based discovery of conformationally selective inhibitors of the serotonin transporter

less than 1 minute read

We recently published “Structure-based discovery of conformationally selective inhibitors of the serotonin transporter” in Cell.

Structural basis of odorant recognition by a human odorant receptor

less than 1 minute read

We recently published “Structural basis of odorant recognition by a human odorant receptor” in Nature.

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2022

Discrete GPCR-triggered endocytic modes enable β-arrestins to flexibly regulate cell signaling

1 minute read

We recently published “Discrete GPCR-triggered endocytic modes enable β-arrestins to flexibly regulate cell signaling” in eLife.

Autoantibody mimicry of hormone action at the thyrotropin receptor

less than 1 minute read

We recently published “Autoantibody mimicry of hormone action at the thyrotropin receptor” in Nature.

New website

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Lauching a new website for the Manglik Lab

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2021

Selective G protein signaling driven by substance P–neurokinin receptor dynamics

less than 1 minute read

We recently published “Selective G protein signaling driven by substance P–neurokinin receptor dynamics” in Nature Chemical Biology.

Biochemical Assays to Directly Assess Smoothened Activation by a Conformationally Sensitive Nanobody

less than 1 minute read

We recently published “Biochemical Assays to Directly Assess Smoothened Activation by a Conformationally Sensitive Nanobody” in Hedgehog Signaling.

Structural insight into SARS-CoV-2 neutralizing antibodies and modulation of syncytia

less than 1 minute read

We recently published “Structural insight into SARS-CoV-2 neutralizing antibodies and modulation of syncytia” in Cell.

Surface Proteomics Reveals CD72 as a Target for In Vitro–Evolved Nanobody-Based CAR-T Cells in KMT2A/MLL1-Rearranged B-ALL

less than 1 minute read

We recently published “Surface Proteomics Reveals CD72 as a Target for In Vitro–Evolved Nanobody-Based CAR-T Cells in KMT2A/MLL1-Rearranged B-ALL” in Cancer ...

CryoEM and AI reveal a structure of SARS-CoV-2 Nsp2, a multifunctional protein involved in key host processes

less than 1 minute read

We recently published “CryoEM and AI reveal a structure of SARS-CoV-2 Nsp2, a multifunctional protein involved in key host processes” in bioRxiv.

Protein design and variant prediction using autoregressive generative models

less than 1 minute read

We recently published “Protein design and variant prediction using autoregressive generative models” in Nature Communications.

Smoothened transduces Hedgehog signals via activity-dependent sequestration of PKA catalytic subunits

less than 1 minute read

We recently published “Smoothened transduces Hedgehog signals via activity-dependent sequestration of PKA catalytic subunits” in PLOS Biology.

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2020

An ultrapotent synthetic nanobody neutralizes SARS-CoV-2 by stabilizing inactive Spike

less than 1 minute read

We recently published “An ultrapotent synthetic nanobody neutralizes SARS-CoV-2 by stabilizing inactive Spike” in Science.

Hedgehog pathway activation through nanobody-mediated conformational blockade of the Patched sterol conduit

less than 1 minute read

We recently published “Hedgehog pathway activation through nanobody-mediated conformational blockade of the Patched sterol conduit” in Proceedings of the Nat...

Universal Polymerase Chain Reaction and Antibody Testing Demonstrate Little to No Transmission of Severe Acute Respiratory Syndrome Coronavirus 2 in a Rural Community

less than 1 minute read

We recently published “Universal Polymerase Chain Reaction and Antibody Testing Demonstrate Little to No Transmission of Severe Acute Respiratory Syndrome Co...

Bi-paratopic and multivalent VH domains block ACE2 binding and neutralize SARS-CoV-2

less than 1 minute read

We recently published “Bi-paratopic and multivalent VH domains block ACE2 binding and neutralize SARS-CoV-2” in Nature Chemical Biology.

Comparative host-coronavirus protein interaction networks reveal pan-viral disease mechanisms

less than 1 minute read

We recently published “Comparative host-coronavirus protein interaction networks reveal pan-viral disease mechanisms” in Science.

Structure of hepcidin-bound ferroportin reveals iron homeostatic mechanisms

1 minute read

We recently published “Structure of hepcidin-bound ferroportin reveals iron homeostatic mechanisms” in Nature.

β-Arrestin–Biased Angiotensin II Receptor Agonists for COVID-19

1 minute read

We recently published “β-Arrestin–Biased Angiotensin II Receptor Agonists for COVID-19” in Circulation.

Test performance evaluation of SARS-CoV-2 serological assays

less than 1 minute read

We recently published “Test performance evaluation of SARS-CoV-2 serological assays” in medRxiv.

Agonist-selective recruitment of engineered protein probes and of GRK2 by opioid receptors in living cells

less than 1 minute read

We recently published “Agonist-selective recruitment of engineered protein probes and of GRK2 by opioid receptors in living cells” in eLife.

Molecular Basis of Opioid Action: From Structures to New Leads

1 minute read

We recently published “Molecular Basis of Opioid Action: From Structures to New Leads” in Biological Psychiatry.

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2019

Smoothened stimulation by membrane sterols drives Hedgehog pathway activity

less than 1 minute read

We recently published “Smoothened stimulation by membrane sterols drives Hedgehog pathway activity” in Nature.

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2018

Development of an antibody fragment that stabilizes GPCR/G-protein complexes

less than 1 minute read

We recently published “Development of an antibody fragment that stabilizes GPCR/G-protein complexes” in Nature Communications.

Measuring ligand efficacy at the mu-opioid receptor using a conformational biosensor

less than 1 minute read

We recently published “Measuring ligand efficacy at the mu-opioid receptor using a conformational biosensor” in eLife.

Structure of the µ-opioid receptor–G_i protein complex

less than 1 minute read

We recently published “Structure of the µ-opioid receptor–Gi protein complex” in Nature.

A Genetically Encoded Biosensor Reveals Location Bias of Opioid Drug Action

less than 1 minute read

We recently published “A Genetically Encoded Biosensor Reveals Location Bias of Opioid Drug Action” in Neuron.

Sortase ligation enables homogeneous GPCR phosphorylation to reveal diversity in β-arrestin coupling

less than 1 minute read

We recently published “Sortase ligation enables homogeneous GPCR phosphorylation to reveal diversity in β-arrestin coupling” in Proceedings of the National A...

Yeast surface display platform for rapid discovery of conformationally selective nanobodies

less than 1 minute read

We recently published “Yeast surface display platform for rapid discovery of conformationally selective nanobodies” in Nature Structural & Molecular Biol...

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2017

Structural Basis for G Protein-Coupled Receptor Activation

less than 1 minute read

We recently published “Structural Basis for G Protein-Coupled Receptor Activation” in Biochemistry.

Nanobodies to Study G Protein–Coupled Receptor Structure and Function

less than 1 minute read

We recently published “Nanobodies to Study G Protein–Coupled Receptor Structure and Function” in Annual Review of Pharmacology and Toxicology.

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2016

Structure-based discovery of opioid analgesics with reduced side effects

less than 1 minute read

We recently published “Structure-based discovery of opioid analgesics with reduced side effects” in Nature.

Allosteric nanobodies reveal the dynamic range and diverse mechanisms of G-protein-coupled receptor activation

1 minute read

We recently published “Allosteric nanobodies reveal the dynamic range and diverse mechanisms of G-protein-coupled receptor activation” in Nature.

Allosteric coupling from G protein to the agonist-binding pocket in GPCRs

1 minute read

We recently published “Allosteric coupling from G protein to the agonist-binding pocket in GPCRs” in Nature.

Crystal structure of the human σ₁ receptor

1 minute read

We recently published “Crystal structure of the human σ1 receptor” in Nature.

High-density grids for efficient data collection from multiple crystals

1 minute read

We recently published “High-density grids for efficient data collection from multiple crystals” in Acta Crystallographica Section D Structural Biology.

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2015

Engineering high-affinity PD-1 variants for optimized immunotherapy and immuno-PET imaging

less than 1 minute read

We recently published “Engineering high-affinity PD-1 variants for optimized immunotherapy and immuno-PET imaging” in Proceedings of the National Academy of ...

Structural insights into µ-opioid receptor activation

less than 1 minute read

We recently published “Structural insights into µ-opioid receptor activation” in Nature.

Propagation of conformational changes during μ-opioid receptor activation

1 minute read

We recently published “Propagation of conformational changes during μ-opioid receptor activation” in Nature.

Structural basis for nucleotide exchange in heterotrimeric G proteins

less than 1 minute read

We recently published “Structural basis for nucleotide exchange in heterotrimeric G proteins” in Science.

Structural Insights into the Dynamic Process of β₂-Adrenergic Receptor Signaling

less than 1 minute read

We recently published “Structural Insights into the Dynamic Process of β2-Adrenergic Receptor Signaling” in Cell.

G Protein-coupled Receptor Kinases of the GRK4 Protein Subfamily Phosphorylate Inactive G Protein-coupled Receptors (GPCRs)

less than 1 minute read

We recently published “G Protein-coupled Receptor Kinases of the GRK4 Protein Subfamily Phosphorylate Inactive G Protein-coupled Receptors (GPCRs)” in Journa...

A comparison of chemical shift sensitivity of trifluoromethyl tags: optimizing resolution in ¹⁹F NMR studies of proteins

less than 1 minute read

We recently published “A comparison of chemical shift sensitivity of trifluoromethyl tags: optimizing resolution in 19F NMR studies of proteins” in Journal o...

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2014

Goniometer-based femtosecond crystallography with X-ray free electron lasers

less than 1 minute read

We recently published “Goniometer-based femtosecond crystallography with X-ray free electron lasers” in Proceedings of the National Academy of Sciences.

Covalent agonists for studying G protein-coupled receptor activation

less than 1 minute read

We recently published “Covalent agonists for studying G protein-coupled receptor activation” in Proceedings of the National Academy of Sciences.

The role of protein dynamics in GPCR function: insights from the β₂AR and rhodopsin

less than 1 minute read

We recently published “The role of protein dynamics in GPCR function: insights from the β2AR and rhodopsin” in Current Opinion in Cell Biology.

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2013

Activation and allosteric modulation of a muscarinic acetylcholine receptor

less than 1 minute read

We recently published “Activation and allosteric modulation of a muscarinic acetylcholine receptor” in Nature.

Applications of molecular replacement to G protein-coupled receptors

less than 1 minute read

We recently published “Applications of molecular replacement to G protein-coupled receptors” in Acta Crystallographica Section D Biological Crystallography.

Adrenaline-activated structure of β₂-adrenoceptor stabilized by an engineered nanobody

1 minute read

We recently published “Adrenaline-activated structure of β2-adrenoceptor stabilized by an engineered nanobody” in Nature.

The Role of Ligands on the Equilibria Between Functional States of a G Protein-Coupled Receptor

1 minute read

We recently published “The Role of Ligands on the Equilibria Between Functional States of a G Protein-Coupled Receptor” in Journal of the American Chemical S...